A Rapid Microbioassay for Discovery of Antagonistic Bacteria for Phytophthora parasitica var. nicotianae
نویسندگان
چکیده
منابع مشابه
Random amplified polymorphic DNA analysis of China isolates of Phytophthora parasitica var. nicotianae from different districts
China isolates of Phytophthora parasitica var. Nicotianae have been divided into three large pathogenic groups (PGS) (Hv(A), Iv(B), Wv(C)) which came from Yunnan, Shandong and Liaoning. These three districts belong to main tobacco regions in China: 15 isolates derived from Yunnan of the southwest, 13 isolates from Shandong of the east and 20 isolates from Liaoning of the northeast. PGS were det...
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A recently isolated cDNA clone of tobacco (Nicotiana tabacum L.) lipoxygenase (LOX) was used to study LOX gene expression in tobacco cell-suspension cultures and intact plants in response to infection with Phytophthora parasitica nicotianae (Ppn). Southern blot analysis of tobacco DNA indicated that only a small number of LOX genes hybridize to this probe. These genes were not constitutively ex...
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The genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by BOXAIR-PCR, 16S-RFLP, 16S rRNA sequence homology and phylogenetic analysis methods. These studies revealed that 4.01% of the 6652 tested had some inhibitory activity against Phytophthora nicotianae. BOXAIR-PCR analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high d...
متن کاملUse of a new tetrazolium-based assay to study the production of superoxide radicals by tobacco cell cultures challenged with avirulent zoospores of phytophthora parasitica var nicotianae
The relationship between the production of reactive oxygen species and the hypersensitive response (HR) of tobacco (Nicotiana tabacum L. ) toward an incompatible race of the Oomycete Phytophthora parasitica var nicotianae has been investigated. A new assay for superoxide radical (O2-) production based on reduction of the tetrazolium dye sodium,3'-(1-[phenylamino-carbonyl]-3, 4-tetrazolium)-bis(...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2012
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto-09-11-0247